The Lab contains large GMP area for cells culture, a molecular biology lab, a cytobiology lab, a histology lab, and area for animal breeding, in which various animal experiments on naked mouse, rat, rabbit, dog, sheep and pig are made. The Lab also owns state-of-the-art equipments such as Micro CT, Altra HyperSort System, Laser Radiation, PixTMLCM, Immune Flsorescence Microscope, and Gel-pro Analyzer.
In recent years, the Lab has succeeded in constructing the tissue engineered cartilage, tendon and skin in vitro. This has laid the foundation for the further development of the tissue engineering commercialization.
Wei Liu, Wenjie Zhang, Guangdong Zhou and Deli Liu
"Tissue engineering: basic research and application of the key technology of tissue construction" won the National Technological Invention Award, Second Prize.
With a focus on the research and development of the core technology of tissue engineering, we have invented a series of tissue construction technologies with independent intellectual property rights; established technical routes such as the skin, tendons, blood vessels, cartilage and bone construction, and completed tissue repair in immunocompetent animal models; and invented bioreactor technology for tendon and blood vessel engineering with independent intellectual property rights. The engineered tissue had good mechanical properties and the ability to repair defects. In addition, we invented new technology for the application of skin fibroblasts, bone marrow stromal cells and allogeneic chondrocytes as seeding cells for the tissue engineering of tendon and cartilage, which expanded the concept and scope of the seeding cells for tissue engineering and provided important parameters for industrialization. These research results have resulted in applications for 35 patents, of which twelve patents (eleven invention patents) have been authorized, resolving a number of key technical issues of the transition of tissue engineering technology from basic research to clinical application. At the same time, we have published 200 journal papers (including 27 SCI indexed papers, with a total IF of 64.93 points, cited 246 times), participated in international conferences as invited speaker for 28 times, and authored or co-authored seven books. Over 60 graduate students have completed their degree programs at our lab. These results have an important impact on the future of tissue engineering research in China and significantly improve the international academic standing of our country. The field of tissue construction has reache an advanced and international level. In addition, the project team has also set up the only National Tissue Engineering Research Center in China, further promoting the industrialization foundation of tissue engineering technology.
In 2005, we began the project of "Tissue engineering: basic and applied basic research" from the International Cooperation of Science Ministry and successfully completed the project in 2008.
During the process of the project, we cooperated with Professor Jeff Hollinger from Carnegie Mellon University in cell culture, purification, identification, and established a culture standard for human bone marrow stem cells and adipose stem cells as well as the effective induction of bone, cartilage, providing sufficient sources of seeding cells. On this basis, we further strengthened the cooperation and exchanges with several other international organizations, including Professor Peter Ma from Michigan University in the United States, Professor Chen Guoping from National Institute for Materials Science, and Professor Antonios Mikos from Rice University, as well as the visiting to Genzyme Corporation and Smith&Nephew company. We also received a number of foreign technicians and students to our laboratory for study exchange.
Through the introduction of foreign technology: we invented the PGA and/or PLA fiber fabrication technology with independent intellectual property rights from the experience of South Korea and Singapore, which greatly reduced material costs of the cartilage and tendon tissue engineering. Also, we learned from Leeds University (UK), designed some bioreactors with independent intellectual property rights, improving the in vitro mechanical function of the engineered tissue; By the introduction of technique from London University School of Medicine, we established a new type of biological gel and cell complex for in vitro two-dimensional and three-dimensional dynamic culture, which provided a good in vitro model for the further study of tissue engineered tissue formation and stem cell differentiation mechanisms; Also, we introduced MicroCT, the image processing system, rapid prototyping systems, three-dimensional laser imaging system, automatic separator beads and other advanced equipment and related software for computer-aided design and precise shape control of the tissue.