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SJTU Team Develops New Technology of Biochip

June 13, 2018      Author: Cheng Li

Tao Shengce Group from Systems Biomedicine Research Institute in cooperation with Dai Junbiao Group of Shenzhen Institutes Of Advanced Technology of Chinese Academy Of Sciences, taking saccharomyces cerevisiae as the research object and using histone marks as examples, created cell lysate microarray for mapping the network of genetic regulators for histone marks. This research results were published online on June 5, 2018 on the Molecular & Cellular Proteomics (MCP).

The paper was published with research fellows Tao Shengce of Shanghai Jiao Tong University Systems Biomedicine Research Institute and Dai Junbiao of Shenzhen Institutes Of Advanced Technology of Chinese Academy Of Sciences as corresponding authors, and PhD student Cheng Li of Tao Shengce Group as the lead author.

The research received support of 'Thirteenth Five-Year Plan' Special Program for Protein Machines and Life Process Regulation and National 'Twelfth Five-Year Plan' Infectious Diseases Major Special Program, as well as National Natural Science Foundation Grant.

Proteins, as the major executer for cell progresses and functions, its abundance and the level of post-translational modifications, are tightly monitored by regulators. Genetic perturbation could help us to understand the relationships between genes and protein functions. Herein, to explore the impact of the genome-wide interruption on certain protein, we developed a cell lysate microarray on kilo-conditions (CLICK) with 4,837 knockout (YKO) and 322 temperature-sensitive (ts) mutant strains of yeast (Saccharomyces cerevisiae). Taking histone marks as examples, a general workflow was established for the global identification of upstream regulators. Through a single CLICK array test, we obtained a series of regulators for H3K4me3, which covers most of the known regulators in S.accharomyces. We also noted that several group of proteins that are involved in negatively regulation of H3K4me3. Further, we discovered that Cab4p and Cab5p, two key enzymes of CoA biosynthesis, play central roles in histone acylation. Because of its general applicability, CLICK array could be easily adopted to rapid and global identification of upstream protein/enzyme(s) that regulate/modify the level of a protein or the posttranslational modification of a non-histone protein.


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